Tetraethylammonium-sensitive apical K+ channels mediating K+ secretion by turtle colon.

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Tetraethylammonium-sensitive apical K+ channels mediating K+ secretion by turtle colon.

1. Apical membrane K+ channels in turtle colon were identified and characterized using current fluctuation analysis. 2. Under short-circuit conditions in NaCl-Ringer solution, the power density spectrum (PDS) of the short-circuit current (Isc) sometimes exhibited a clearly discernible Lorentzian component, indicating spontaneous fluctuations produced by a population of apical ion channels. The ...

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The exocrine salivary glands of mammals secrete K+ by an unknown pathway that has been associated with HCO3(-) efflux. However, the present studies found that K+ secretion in the mouse submandibular gland did not require HCO3(-), demonstrating that neither K+/HCO3(-) cotransport nor K+/H+ exchange mechanisms were involved. Because HCO3(-) did not appear to participate in this process, we tested...

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Quantification of K+ secretion through apical low-conductance K channels in the CCD.

Outward and inward currents through single small-conductance K(+) (SK) channels were measured in cell-attached patches of the apical membrane of principal cells of the rat cortical collecting duct (CCD). Currents showed mild inward rectification with high [K(+)] in the pipette (K(p)(+)), which decreased as K(p)(+) was lowered. Inward conductances had a hyperbolic dependence on K(p)(+) with half...

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Regulation of AMPA receptor currents by mitochondrial ATP-sensitive K+ channels in anoxic turtle neurons.

Mammalian neurons rapidly undergo excitotoxic cell death during anoxia, whereas neurons from the anoxia-tolerant painted turtle survive without oxygen for hours and offer a unique model to study mechanisms to reduce the severity of cerebral stroke. An anoxia-mediated decrease in whole cell N-methyl-D-aspartate receptor and α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor (AMPAR) cu...

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Role of 20-HETE in mediating the effect of dietary K intake on the apical K channels in the mTAL.

We have used the patch-clamp technique to study the effect of dietary K intake on the apical K channels in the medullary thick ascending limb (mTAL) of rat kidneys. The channel activity, defined by the number of channels in a patch and the open probability (NPo), of the 30- and 70-pS K channels, was 0.18 and 0.11, respectively, in the mTAL from rats on a K-deficient diet. In contrast, NPo of th...

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ژورنال

عنوان ژورنال: The Journal of Physiology

سال: 1993

ISSN: 0022-3751

DOI: 10.1113/jphysiol.1993.sp019577